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LU Kehuan, PhD

Tenured Professor

Tel/Fax: 86-771-3238064

Email: khlu@gxu.edu.cn

Website at GXU: http://210.36.22.97/tcms/teachInfo!queryTeachInfoById.action?gh=FCCBDC48118B11E69C550050569949B5

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Careers & Education

  • 1987.12 - Current, Professor, Director

       Animal Reproduction Institute, College of Animal Science and Techonlogy, 

       SKL for Conservation & Utilization of Subtropical Agro-bioresource,

       Guangxi University, Nanning, China, 530004

  • 1987.11 - 2001.11 Senior Research Scientist, R&D Director

       Ovamass Inc. (Ireland), ABC Inc. (UK), XY Inc. (USA)

  • 1985.08 - 1990.07, Ph.D

       University College Dublin, Belfield, Dublin 4, Ireland

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Publications

  1. Stephens S., Lu K.H., Boland M.P., Gordon I. Fertilization of pig oocytes in vivo and in vitro. Theriogenology, 1986. 25: 203.

  2. Gordon I., Lu K.H., Boland M.P., Crosby T.F. In vitro fertilization of bovine oocytes matured in vitro. Animal Production, 1987. 44: 468.

  3. Lu K.H., Boland M.P., Crosby T.F., Gordon I. In vitro fertilization of cattle oocytes matured in vitro  Theriogenology, 1987. 27: 51.

  4. Lu K.H., Boland M.P., Gordon I. In vitro fertilization of pig oocytes matured in vivo. Theriogenology, 1987. 27: 252.

  5. Lu K.H., Gordon I. Effect of pH on capacitation and fertilization medium on the in vitro fertilization of bovine oocytes  Proc Soc Study of Fert (York), 1987: 77.

  6. Lu K.H., Gordon I. Effect of serum, hormones and cumulus cells on the in vitro maturation of bovine oocytes.   . Proc Soc Study of Fert (York), 1987: 81.

  7. Lu K.H., Gordon I., Boland M.P., Crosby T.F. In vitro fertilization of bovine oocytes matured In vitro. Proc 92nd Meeting of BSAP (Scarborough), 1987: 28.

  8. Lu K.H., Gordon I., Boland M.P., Crosby T.F., McGovern H., Gallagher M. Utilization of ovarian oocytes derived from slaughtered heifers in the production of embryos for cattle twining   13th Ann Research Meeting of Irish Grassland and Animal Production Association, 1987: 7-8.

  9. Lu K.H., Gordon I., Chen H.B., McGovern H. In vitro culture of early bovine embryos derived from in vitro fertilization of follicular oocytes matured in vitro. Proc 3rd Scientific Meeting of European Embryo Transfer Association (Lyon), 1987: 169.

  10. Lu K.H., Gordon I., Gallagher M., McGovern H. Pregnancy established in cattle by transfer of embryos derived from in vitro fertilisation of oocytes matured in vitro. Vet Rec, 1987. 121(11): 259-60.

  11. Lu K.H., McGovern H., Gallagher M., MacDonnell H.F., Gordon I. Birth of calves after in vitro maturation and fertilization of oocytes and the in vivo/in vitro culture of zygotes  13th Annual Research Meeting of Irish Grassland and Animal Production Association, 1987.

  12. Gordon I., Lu K.H., Gallagher M., McGovern H. Production of cattle embryos by in vitro and in vivo culture. Animal Production, 1988. 46: 498.

  13. Lu K.H., Gordon I. Effect of heparin on the capacitation of frozen-thawed bovine spermatozoa used in the in vitro fertilization of oocytes matured in vitro. Proc 11th Int Congr Anim Reprod and AI（Dublin）, 1988. 3: 339.

  14. Lu K.H., Gordon I., Chen H.B., Gallagher M., McGovern H. Birth of twins after transfer of cattle embryos produced by in vitro techniques. Vet Rec, 1988. 122(22): 539-40.

  15. Lu K.H., Gordon I., McGovern H. Factors affecting in vivo development of in vitro fertilized cattle eggs in relation to follicle size in donor ovary and time of transfer to sheep oviduct. Proc 11th Int Congr Anim Reprod and AI（Dublin）, 1988. 3: 341.

  16. Lu K.H., Gordon I., McGovern H., Gallagher M. Development in vitro of bovine morulae derived from in vitro fertilization of follicular oocytes matured in vitro  Proc 11th Int Congr Anim Reprod and AI（Dublin）, 1988. 3: 340.

  17. Lu K.H., Gordon I., McGovern H., Gallagher M. Pregnancy established in cattle after transfer of frozen-thawed bovine embryos derived from in vitro fertilization of follicular oocytes matured in vitro  Proc 11th Int Congr Anim Reprod and AI（Dublin）, 1988. 3: 342.

  18. Lu K.H., Gordon I., McGovern H., Gallagher M. Production of cattle embryos by in vitro maturation and fertilization of follicular oocytes and their subsequent culture in vivo in sheep Theriogenology, 1988. 29: 272.

  19. Jiang H.S., Wang W.L., Lu K.H., McCarthy D., Gordon I. In vitro culture of early bovine embryos derived from in vitro fertilization, comparison with in vivo culture. J Reprod Fert, 1989. 3: 89.

  20. Lu K.H., MacDonnell H.F., Gordon I. Birth of calves after in vitro maturation and fertilization of follicular oocytes. Theriogenology, 1989. 31: 222.

  21. Wang W.L., Jiang H.S., Lu K.H., McCarthy D., Gordon I. The effect of media and sera on the in vitro development of early bovine embryos. J Reprod Fert, 1989. 3: 50.

  22. Chen-Lu H.B., Lu K.H. Effect of protein supplements on in vitro maturation, fertilization and culture to blastocyst and hatching stage of bovine oocytes. Theriogenology, 1990. 33: 205.

  23. Gordon I., Lu K.H. Production of embryos in vitro and its impact on livestock production. Theriogenology, 1990. 33: 77-87.

  24. Jiang H.S., Wang W.L., Lu K.H., Gordon I. Effects of PMSG, insulin, osmolality and oestrous cow serum on development of IVF early bovine embryos cultured on granulosa cell monolayers. Theriogenology, 1990. 33: 258.

  25. Ling Z.J., Lu K.H. Frequency of cleavage and development in vitro of bovine oocytes fertilized in different numbers in drops with different sperm concentrations. Theriogenology, 1990. 33: 275.

  26. Lu K.H., Jiang H.S., Wang W.L., Gordon I. Pregnancies established in cattle by transfer of fresh and frozen embryos derived from in vitro maturation and fertilization of oocytes and their subsequent culture in vitro. Theriogenology, 1990. 33: 278.

  27. Shi D.S., Lu K.H., Gordon I. Effects of bulls on fertilization of bovine oocytes and their subsequent development in vitro. Theriogenology, 1990. 33: 324.

  28. Tan S.J., Lu K.H. Effects of different oestrous stages of ovaries and sizes of follicles on generation of bovine embryos in vitro. Theriogenology, 1990. 33: 335.

  29. Wang W.L., Jiang H.S., Lu K.H., Gordon I. Effect of condition medium and glucose concentration on the in vitro development of early bovine embryos. Theriogenology, 1990. 33: 343.

  30. Yang N.S., Lu K.H., Gordon I. In vitro fertilization (IVF) and culture (IVC) of bovine oocytes from stored ovaries. Theriogenology, 1990. 33: 352.

  31. Yang Y.B., Lu K.H. The influence of bovine oocyte type on in vitro fertilization and subsequent development in vitro. Theriogenology, 1990. 33: 355.

  32. Chen-Lu H.B., Huang F.L., Lu K.H., Polge C. Effect of oviductal cell suspensions on in vitro fertilization and development of bovine oocytes. Proc 7th Scientific Meeting of European Embryo Transfer Association （Cambridge）, 1991: 134.

  33. Jiang H.S., Wang W.L., Lu K.H., Gordon I., Polge C. Roles of different cell monolayers in the co-culture of IVF bovine embryos. Theriogenology, 1991. 35: 216.

  34. Lu K.H., Polge C. Pregnancy and twinning rates after transfer of IVF embryos to the bred recipients. Proc 7th Scientific Meeting of European Embryo Transfer Association （Cambridge）, 1991: 164.

  35. Lu K.H., Shi D.S., Jiang H.S., Goulding D., Boland M.P., Roche J.F. Comparison of the developmental capacity of bovine oocytes from superovulated and non stimulated heifers. . Theriogenology, 1991. 35: 234.

  36. Polge C., Broadbent P., Lu K.H. Pregnancy rates following transfer of fresh or frozen bovine embryos produced in vitro. International Symposium on Animal Biotechnology (Kyoto）, 1991: 5.

  37. Shi D.S., Lu K.H., Gordon I., Polge C. Variation in cleavage and embryonic development of bovine oocytes in vitro fertilized with different bull ejaculates. Theriogenology, 1991. 35: 271.

  38. Wang W.L., Jiang H.S., Shi D.S., Lu K.H., Gordon I., Polge C. The effect of temperature on the in vitro maturation, fertilization and development of bovine oocytes. Proc 7th Scientific Meeting of European Embryo Transfer Association（Cambridge）, 1991: 216.

  39. Yang N.S., Duff R., Lu K.H., Gordon I., Polge C. Effect of storage temperature and time on the viability of bovine embryos produced in vitro. Theriogenology, 1991. 35: 297.

  40. Chen H.B., Lu K.H., Polge C. In vitro fertilization (IVF) of bovine oocytes with frozen-thawed sperm after storage at different temperatures and periods of time. 12th International Congress on Animal Reproduction（Hague, Netherlands）, 1992: 629-631.

  41. Huang F.L., Lu K.H., Polge C. Effect of sucrose concentration on viability of bovine IVF embryos frozen by a rapid method. 12th International Congress on Animal Reproduction（Hague, Netherlands）, 1992: 1430-1432.

  42. Jiang H.S., Wang W.L., Lu K.H., Gordon I., Polge C. Examination of cell numbers of blastocysts derived from IVM, IVF and IVC of bovine follicular oocytes. Theriogenology, 1992. 37: 229.

  43. Li X.F., Wu G.W., Tan L.L., Tan S.J., Lu K.H. Effects of electrical pulses on the nuclear transfer of rabbit oocytes matured in vivo/in vitro. 12th International Congress on Animal Reproduction（Hague, Netherlands）, 1992: 709-711.

  44. Lu K.H., Polge C. A summary of two-year's results in large scale in vitro bovine embryo production. 12th International Congress on Animal Reproduction（Hague, Netherlands）, 1992: 1315-1317.

  45. McCaffrey C., Lu K.H., Sreenan J.M. Development of in vivo and in vitro fertilised (IVF) cattle ova during in vitro culture. 12th International Congress on Animal Reproduction（Hague, Netherlands）, 1992: 366-368.

  46. McCaffrey C., Lu K.H., Sreenan J.M. Factors involved in the in vitro development of IVF cattle ova. Proceedings of 18th Annual Research Meeting of the Irish Grassland and Animal Production Association（Dublin）, 1992: 33-34.

  47. Wang W.L., Jiang H.S., Lu K.H., Gordon I., Polge C. The effect of gas phase on the in vitro development of bovine embryos derived from in vitro maturation and fertilization of ovarian oocytes. Theriogenology, 1992. 37: 320.

  48. Yang N.S., Lu K.H., Gordon I., Polge C. Vitrification of bovine blastocysts produced in vitro. Theriogenology, 1992. 37: 326.

  49. Yang N.S., Lu K.H., Polge C. Effect of equilibration temperature and time on the survival of vitrified in vitro bovine blastocysts. 12th International Congress on Animal Reproduction（Hague, Netherlands）, 1992: 1505-1507.

  50. Cran D.G., Cochrane D.J., Johnson L.A., Wei H., Lu K.H., Polge C. Separation of X and Y-chromosome bearing bovine sperm by flow cytometry for use in IVF. Theriogenology, 1994. 41: 183.

  51. Lu K.H., Cran D.G., Seidel G.E. In vitro fertilization with flow-cytometrically-sorted bovine sperm. Theriogenology, 1999. 52(8): 1393-1405.

  52. Zhang M., Lu K.H., Seidel G.E. Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically sorted, stained and unsorted sperm from different bulls. Theriogenology, 2003. 60(9): 1657-63.

  53. Lu K.H., Seidel G.E., Jr. Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm. Theriogenology, 2004. 62(5): 819-30.

  54. Lu S.S., Yang X.R., Zhang M., Li Z.Q., Jiang H.S., Lu K.H. Effects of porcine follicular fluid and fetal calf serum on in vitro maturation of porcine oocytes derived from small follicles. Proceedings of the XIXth International Congress of Zoology. Beijing (China), 2004: 340.

  55. Lu S.S., Wei F., Fang H.L., Zhang M., Lu K.H. Effects of different chemical activation protocols on early parthenogenetic development of porcine oocytes. Proceedings of 5th International Conference on Transgenic Animals, Guangzhou (China), 2005: 114.

  56. Zhang M., Lu Y.Q., Lu S.S., Lu K.H. Chromosomal analysis of bovine blastocysts produced by ICSI and IVF with sorted sperms. Proceedings of 5th International Conference on Transgenic Animals, Guangzhou (China), 2005: 171.

  57. Lu S.S., Liu H.B., Lv P.R., Luo L.X., Lu Y.Q., Zhang M., Wang A.D., Lu K.H. Effect of different electro-activation parameters on parthenogenetic development of porcine oocytes. The 3rd Annual Conference of Asian Reproductive Biotechnology Society. Hanoi (Vietnam), 2006: 13.

  58. Lu Y.Q., Wang W.L., Yang H., Liang X.W., Liang Y.Y., Zhang M., Lu S.S., Kitiyanant Y., Lu K.H. Flow-cytometric sorting the sperm and production of sex-preselected embryo in buffalo (Bubalus Bubalis). Proceedings of the 5th Asian Buffalo Congress, Nanning (China), 2006: 155-161.

  59. Lu Y.Q., Zhang M., Meng B., Lu S.S., Wei Y.M., Lu K.H. Identification of X- and Y-chromosome bearing buffalo (Bubalus bubalis) sperm. Anim Reprod Sci, 2006. 95(1-2): 158-64.

  60. Fu Q., Zhang M., Qin W.S., Lu Y.Q., Zheng H.Y., Meng B., Lu S.S., Lu K.H. Cloning the swamp buffalo SRY gene for embryo sexing with multiplex-nested PCR. Theriogenology, 2007. 68(9): 1211-8.

  61. Liu H.B., Lu S.S., Lv P.R., Yang X.G., Qin X.E., Pi D.Y., Xu D.N., Li L., Lu Y.Q., Lu K.H. Preliminary study on inter-species nuclear transfer using buffalo (Bubalus Bubalis) and human somatic cells as donor and buffalo and porcine cytoplast as recipient. The 4th Annual Conference of Asian Reproductive Biotechnology Society, Singapore, 2007: 10-12.

  62. Lu S.S., Lv P.R., Liu H.B., Yang X.G., Qin X.E., Pi D.Y., Xu D.N., Ren Z.L., Lu Y.Q., Lu K.H. Somatic cell nuclear transfer in Guangxi Bama miniature pig (Sus scrofa domestica). The 4th Annual Conference of Asian Reproductive Biotechnology Society. Singapore, 2007: 84-87.

  63. Lu Y.Q., Liang X.W., Zhang M., Wang W.L., Kitiyanant Y., Lu S.S., Meng B., Lu K.H. Birth of twins after in vitro fertilization with flow-cytometric sorted buffalo (Bubalus bubalis) sperm. Anim Reprod Sci, 2007. 100(1-2): 192-6.

  64. Zhang M., Fu Q., Qin W.S., Zheng H.Y., Lu Y.Q., Lu S.S., Lu K.H. Amplification of the SRY gene for sexing of buffalo (Bubalus bubalis) embryos using nested multiplex PCR. Reproduction Fertility and Development, 2007. 19(1): 300-300.

  65. Liang X.W., Lu Y.Q., Chen M.T., Zhang X.F., Lu S.S., Zhang M., Pang C.Y., Huang F.X., Lu K.H. In vitro embryo production in buffalo (Bubalus bubalis) using sexed sperm and oocytes from ovum pick up. Theriogenology, 2008. 69(7): 822-6.

  66. Liang X.W., Zhu J.Q., Miao Y.L., Liu J.H., Wei L., Lu S.S., Hou Y., Schatten H., Lu K.H., Sun Q.Y. Loss of methylation imprint of Snrpn in postovulatory aging mouse oocyte. Biochem Biophys Res Commun, 2008. 371(1): 16-21.

  67. Lu S.S., Zuo E.W., Wang X.W., Pi D.Y., Lu K.H. Molecular cloning and analysis of E-cadherin gene from porcine oviduct. The 5th Annual Conference of Asian Reproductive Biotechnology Society. Kunming (China), 2008: 76.

  68. Qiu H.B., Lu S.S., Ji K.L., Song X.M., Lu Y.Q., Zhang M., Lu K.H. Membrane progestin receptor beta (mPR-beta): a protein related to cumulus expansion that is involved in in vitro maturation of pig cumulus-oocyte complexes. Steroids, 2008. 73(14): 1416-23.

  69. Zhang M., Niu J.T., Lu Y.Q., Fu Q., Qin W.S., Ren Z.L., Lu S.S., Lu K.H. Cloning and analyzing the 5 ' region of follicle stimulating hormone receptor (FSHR) gene on Swamp Buffalo. Reproduction in Domestic Animals, 2008. 43: 93-93.

  70. Zhuang X.J., Zhang M., Lu Y.Q., Tang J.W., Lu S.S., Lu K.H. Sex determination of bovine embryos biopsies using fluorescence in situ hybridization. The 5th Annual Conference of Asian Reproductive Biotechnology Society. Kunming (China), 2008: 61.

  71. Liu H.B., Lv P.R., Yang X.G., Qin X.E., Pi D.Y., Lu Y.Q., Lu K.H., Lu S.S., Li D.S. Fibroblasts from the new-born male testicle of Guangxi Bama mini-pig ( Sus scrofa) can support nuclear transferred embryo development in vitro. Zygote, 2009. 17(2): 147-56.

  72. Lu S.S., Liu H.B., Lv P.R., He R.G., Qin X.E., Pan T.B., Lan H.E., Huang M.R., Lu K.H. Cloned Guangxi Bama Mini-Pig (Sus Scrofa) Generated from Cultured New-Born Male Gonad Fibroblast Cells is Fertile. The 6th Annual Conference of Asian Reproductive Biotechnology Society. Siem Reap (Cambodia), 2009: 54.

  73. Lu Y.Q., Ye D.N., Zhang M., Lu S.S., Lu K.H. Leptin Enhanced the Development of Buffalo (Bubalus Bubalis) Embryo Cultured in Vitro. Reproduction Fertility and Development, 2009. 21(1): 159-160.

  74. Zhang M., Zhuang X.J., Lu Y.Q., Hu C.H., Lu S.S., Lu K.H. Identification of X- and Y-Bearing Spermatozoa in Sorted Buffalo (Bubalus Bubalis) Semen by Fluorescence in Situ Hybridization. Reproduction Fertility and Development, 2009. 21(1): 231-232.

  75. Liu H.B., Lv P.R., He R.G., Yang X.G., Qin X.E., Pan T.B., Huang G.Y., Huang M.R., Lu Y.Q., Lu S.S., Li D.S., Lu K.H. Cloned Guangxi Bama Minipig (Sus scrofa) and Its Offspring Have Normal Reproductive Performance. Cellular Reprogramming, 2010. 12(5): 543-550.

  76. Lu Y., Zhang M., Lu S., Xu D., Huang W., Meng B., Xu H., Lu K. Sex-preselected buffalo (Bubalus bubalis) calves derived from artificial insemination with sexed sperm. Anim Reprod Sci, 2010. 119(3-4): 169-71.

  77. Ning S.F., Lu S.S., Li Q.Y., Hu L.L., Lu K.H. De-differentiation of Swine Testical Cells by the Transcription Factor Oct-4. The 7th Annual Conference of Asian Reproductive Biotechnology Society. Kuala Lumpur (Malaysia), 2010: 48.

  78. Xu C.L., Lu S.S., Qin Z.X., Liang M.M., Luo X., Luo J., Lu K.H. Effect of dimethyl sulfoxide on cell cycle synchronization of in vitro cultured Maccaca Fascicularis ear skin fibroblasts for somatic cell Nuclear Transfer. The 7th Annual Conference of Asian Reproductive Biotechnology Society. Kuala Lumpur (Malaysia), 2010: 32.

  79. Hu C.H., Wang X.W., Lu S.S., Zhang M., Lu Y.Q., Yang X.G., Lu K.H. Tacrine can significantly improve motility of frozen-thawed boar spermatozoa. Reproduction in Domestic Animals, 2011. 46: 92-92.

  80. Hu C.H., Zhuang X.J., Wei Y.M., Zhang M., Lu S.S., Lu Y.Q., Yang X.G., Lu K.H. Freezing procedure can result in more serious apoptosis of boar spermatozoa than bull spermatozoa. Reproduction in Domestic Animals, 2011. 46: 93-93.

  81. Li Q.Y., Song X.M., Li D., Yang X.G., Lu S.S., Lu Y.Q., Zhang M., Xu H.Y., Lu K.H. Inhibition of buffalo oocyte meiosis by meiotic inhibitors during in vitro maturation. The 8th Annual Conference of Asian Reproductive Biotechnology Society.  Guilin (China), 2011: 90.

  82. Li X.X., Yang X., Lu S., Lu Y., Zhang M., Wang M., Chen H., Li Q., Lu K. Effects of melatonin against oxidative stress on flow cytometry sorted buffalo sperm and subsequent in vitro embryo development. Reproduction in Domestic Animals, 2011. 46: 122-123.

  83. Ning S.F., Lu S., Li Q., Liang M., Yang X., Lu Q., Lu K. Methylation characteristic and developmental potential of guangxi bama minipig (Sus scrofa domestica) cloned embryos from donor cells treated with trichostatin A and 5-aza-2 '-deoxycytidine. Reproduction in Domestic Animals, 2011. 46: 134-135.

  84. Yang X.G., Lu S., Wang X., Zuo E., Lu Y., Lu K. cDNA cloning of porcine E-cadherin and its expression profile in porcine early parthenotes. Reproduction in Domestic Animals, 2011. 46: 160-160.

  85. Zeng Y.Q., Yang X.G., Lu Y.Q., Xu H.Y., Lu S.S., Zhang M., Lu K.H. Sex-preselected piglets derived from surgical artificial insemination with sexed sperm. Reproduction in Domestic Animals, 2011. 46: 109-109.

  86. Zhu Z.Y., Yang X.G., Zuo E.W., Zhang W.X., Lu S.S., Zhang M., Lu K.H. Establishment of a fast, invasive and intelligent detection system to detect different concentrations of NaOH in mouse sperm. The 8th Annual Conference of Asian Reproductive Biotechnology Society.  Guilin (China),, 2011: 48.

  87. Zhuang X.J., Lu Y.Q., Zhang M., Lu S.S., Lu K.H. Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa. Anim Reprod Sci, 2011. 126(1-2): 32-6.

  88. Zhu Z.Y., Yang X.G., Zuo E.W., Zhang W.X., Lu S.S., Zhang M., Lu K.H. A model of peptides expression in buffalo embryonic metabolism fluid at preimplantation with MALDI-TOF mass spectrometry. The 8th Annual Conference of Asian Reproductive Biotechnology Society.  Guilin (China),, 2011, Oct 25-28: 70.

  89. Li X.X., Yang X.G., Lu Y.Q., Lu S.S., Zhang M., Yao H.I., Meng L.J., Lu K.H. Protective effects of melatonin against oxidative stress in flow cytometry-sorted buffalo sperm. Reprod Domest Anim, 2012. 47(2): 299-307.

  90. Luo X., Zuo E., Li Q., Yang X., Lu S., Lu Y., Zhang M., Lu K. Melatonin enhances buffalo oocyte maturation and embryo development in vitro. Reproduction in Domestic Animals, 2012. 47: 94-95.

  91. Song X., Yang X., Jiang M., Meng W., Li Q., Li D., Lu Y., Zhang M., Lu S., Lu K. Sorted sperm decreased the sperm quality and subsequent embryonic development after in vitro fertilization of oocytes in buffalo. Reproduction in Domestic Animals, 2012. 47: 109-109.

  92. Song X.M., Lu S.S., Wang M., Li Q.Y., Li D., Yang X.G., Lu Y.Q., Zhang M., Lu K.H. Addition of Glutathione to Semen Extender During Sperm Sorting can Improve in vitro Embryonic Development and in vivo Fertility in Buffalo. Journal of Animal and Veterinary Advances, 2012. 11(23): 4482-4488.

  93. Yang H., Zhang W., Luo F., Zhao H., Hu L., Zuo E., Lu S., Wu Y., Lu K. Isolation, cultivation and identification of spermatogonial stem cells from adult buffalo testis. Reproduction in Domestic Animals, 2012. 47: 114-114.

  94. Yang X.G., Wang X.W., Zuo E.W., Hao C.L., Lu S.S., Liu H.B., Xu H.Y., Lu Y.Q., Lu K.H. cDNA cloning of porcine CDH1 and its expression profile in porcine early parthenotes. Animal Science Papers and Reports, 2012. 30(3): 249-259.

  95. Zhang M., Chen H.H., Tang J.W., Liang X.W., Chen M.T., Qin G.S., Fu Q., Zhang X.F., Zheng H.Y., Lu K.H. Birth of First Buffalo (Bubalus Bubals) Calf Following Embryo Splitting and Polymerase Chain Reaction Sexing at Blastocyst Stage. Reproduction Fertility and Development, 2012. 24(1): 164-165.

  96. Zhang M., Huang Y., Chen H., Lu K. Effects of brilliant cresyl blue staining on the efficiency of in vitro fertilization and parthenogenetic activation of oocytes in buffalo (Bubalus bubalis). Reproduction in Domestic Animals, 2012. 47: 515-515.

  97. Zhuang X.J., Huang Y., Duan Y.P., Zhang M., Lu Y.Q., Lu K.H. Translocation of active mitochondria during buffalo (Bubalus bubalis) oocytes in vitro maturation, fertilization and preimplantation embryo development. Reprod Domest Anim, 2012. 47(3): 443-8.

  98. Hu L.L., Lou J., Zhu X.X., Quan S.N., Lu S.S., Lu K.H. Effects of serum starvation, roscovitine treatment and contact inhibition on the cell-cycle synchronization of new-born Bama mini-pig transgenic kidney fibroblast cells and the development of transgenic cloned embryos. The 10th Annual Conference of Asian Reproductive Biotechnology Society.  Phan Thiet (Vietnam), 2013: 83.

  99. Li D., Lou J., Zhang W.X., Yang X.G., Lu Y.Q., Lu K.H. A Two-step Culture System Sustains Individual Buffalo Embryo Development. Buffalo Bulletin, 2013. 32: 401-404.

  100. Lu K.H., Lu Y.Q., Zhang M., Yang X.G. Sperm Sexing in Buffalo Using Flow Cytometry. Buffalo Bulletin, 2013. 32: 22-30.

  101. Ning S.F., Li Q.Y., Liang M.M., Yang X.G., Xu H.Y., Lu Y.Q., Lu S.S., Lu K.H. Methylation characteristics and developmental potential of Guangxi Bama minipig (Sus scrofa domestica) cloned embryos from donor cells treated with trichostatin A and 5-aza-2 '-deoxycytidine. Zygote, 2013. 21(2): 178-186.

  102. Xu C.L., Qin Z.X., Liang M.M., Chen H.H., Yang L., Luo X., Luo J., Xu H.Y., Cui J.H., Lu S.S., Lu K.H. Effect of Dimethyl Sulfoxide on Cell Cycle Synchronization of in vitro Cultured Monkey (Maccaca fascicularis) Ear Skin Fibroblasts. Journal of Animal and Veterinary Advances, 2013. 12(2): 242-247.

  103. Zheng X.B., Cen M., Wang L.X., Xin G.Y., Fu X.H., Liu P., Li G.H., Zhang M., Lu S.S., Lu K.H. Cloning, Expression and Purification of Nanog Protein from Capra hircus in Escherichia coli. Journal of Animal and Veterinary Advances, 2013. 12(2): 201-207.

  104. Huanga D.L., Fu Q., Wang Z.Q., Huang Y.L., Guan J.L., Lu K.H., Zhang M. The Protein Profile of Buffalo Testis. Current Proteomics, 2014. 11(4): 245-251.

  105. Liu H.B., Lv P.R., Zhu X.X., Wang X.W., Yang X.G., Zuo E.W., Lu Y.Q., Lu S.S., Lu K.H. In vitro development of porcine transgenic nuclear-transferred embryos derived from newborn Guangxi Bama mini-pig kidney fibroblasts. In Vitro Cellular & Developmental Biology-Animal, 2014. 50(9): 811-821.

  106. Zhu X.X., Quan S.N., Zeng Y.L., Huang Y., Sun R.Y., Lu K.H., Lu S.S. Efficient establishment of Guangxi Bama mini-pig transgenic fibroblasts via Xfect polymer transfection. Romanian Biotechnological Letters, 2014. 19(6): 9883-9890.

  107. Hu L.L., Lu Y.Q., Xu H.Y., Yang X.G., Lu S.S., Lu K.H. Production of hGFAP-DsRed transgenic Guangxi Bama mini-pigs via somatic cell nuclear transfer. Genetics and Molecular Research, 2015. 14(4): 16285-16296.

  108. Huang Y.L., Fu Q., Yang L., Guan J.L., Pan H., Chen F.M., Lu K.L., Zhang M. Differences between high- and low-motility buffalo sperm identified by comparative proteomics. Reprod Domest Anim, 2015. 50(3): 443-51.

  109. Lu Y.Q., Liao Y.Q., Zhang M., Yang B.Z., Liang X.W., Yang X.G., Lu S.S., Wu Z.Y., Xu H.Y., Liang Y.B., Lu K.H. A field study on artificial insemination of swamp and crossbred buffaloes with sexed semen from river buffaloes. Theriogenology, 2015. 84(6): 862-867.

  110. Zuo E.W., Yang X.G., Lu Y.Q., Xie L., Shang J.H., Li D., Yang H., Hu L.L., Zhao H.M., Lu S.S., Lu K.H. ZPAC Is Required for Normal Spermatogenesis in Mice. Molecular Reproduction and Development, 2015. 82(10): 747-755.

  111. 11Bai L., Li M., Sun J., Yang X., Lu Y., Lu S., Lu K. RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming. PLoS One, 2016. 11(4): e0153093.

  112. Chen D., Chen M., Lu Z., Yang M., Xie L., Zhang W., Xu H., Lu K., Lu Y. Cholesterol induces proliferation of chicken primordial germ cells. Anim Reprod Sci, 2016. 171: 36-40.

  113. Huang G.B., Quan L., Zeng Y.L., Yang J., Lu K.H., Lu S.S. Role of linker histone H1c during the reprogramming of Chinese swamp buffalo (Bubalus Bubalis) embryos produced by somatic cell nuclear transfer. Reprod Fertil Dev, 2016. 28(3): 302-9.

  114. Li X.X., Wang M., Chen H.H., Li Q.Y., Yang H., Xu H.Y., Lu Y.Q., Zhang M., Yang X.G., Lu S.S., Lu K.H. Flow cytometric and near-infrared Raman spectroscopic investigation of quality in stained, sorted, and frozen-thawed buffalo sperm. Anim Reprod Sci, 2016. 170: 90-9.

  115. Wang C.L., Xu H.Y., Xie L., Lu Y.Q., Yang X.G., Lu S.S., Lu K.H. Stability of the cytoskeleton of matured buffalo oocytes pretreated with cytochalasin B prior to vitrification. Cryobiology, 2016. 72(3): 274-82.

  116. Zhang W.X., Zuo E.W., He Y., Chen D.Y., Long X., Chen M.J., Li T.T., Yang X.G., Xu H.Y., Lu S.S., Zhang M., Lu K.H., Lu Y.Q. Promoter structures and differential responses to viral and non-viral inducers of chicken melanoma differentiation-associated gene 5. Mol Immunol, 2016. 76: 1-6.

  117. Zhu X., Nie J., Quan S., Xu H., Yang X., Lu Y., Lu K., Lu S. In vitro production of cloned and transgenically cloned embryos from Guangxi Huanjiang Xiang pig. In Vitro Cell Dev Biol Anim, 2016. 52(2): 137-43.

  118. Zhu X.X., Quan S.N., Nie J.Y., Lu K.H., Lu S.S. Human glial fibrillary acidic protein gene promoter targets hepatic and pancreatic stellate cells in transgenic Bama mini-pigs. Pakistan Journal of Zoology, 2016. 48(1): 235-240.

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